Fig. 2

Relative quantitative qRT-PCR analysis for mRNA expression of FUT8 in wild-type (LNCaP and LAPC4) and in androgen-resistant LNCaP-95 and LAPC4-AI prostate cancer cells. Error bars represent mean ± SE and * indicates P ≤ 0.05 (a). Western blot analysis of FUT8 in LAPC4-WT and LAPC4-AI prostate cancer cells (b). Western blot analysis of FUT8 in LAPC4-WT cells treated with or without bicalutamide (10 µM) for 72 h (c). Confocal microscopy for the AAL lectin in LAPC4-WT or bicalutamide (cdx) treated or LAPC4-AI and LAPC4-AI treated with 10 µM of bicalutamide (d) (online color figure)