Fig. 2
From: Detection of PRMT1 inhibitors with stopped flow fluorescence
Time course of PRMT1 methylation (1–900 s). The raw data (total of 10,000 points) are shown as blue dots. The curve fitted by equation 2 is shown as a solid black line, which is the average of 4 or 5 replicates. A magnified view of 1–100 s is shown on the side. The concentrations of the enzyme, cofactor and substrate are as follows: [PRMT1] = 0.2 µM, [SAM] = 3.5 µM, [H4FL] = 0.4 µM
