Fig. 3
From: Detection of PRMT1 inhibitors with stopped flow fluorescence
Time course of PRMT1 methylation with varied enzyme concentrations. a The curves were fitted with equation 2 to generate the values in Table S1A. Each fitting curve used 10,000 data points, but only 50 data points are shown. Each curve is the average of 4 to 6 replicates. b shows the simulation results from the values in Table S1A at fixed c = 1. c, d represent the relationship of the slope values (a·k1 and b·k2) with varying concentrations of PRMT1 (values listed in Table S1B). The linear fitting curves are shown as solid black lines. The concentrations of the cofactor and the substrate were fixed at [SAM] = 3.5 µM and [H4FL] = 0.4 µM in these experiments
