Fig. 5

Stopped flow fluorescence assay of the cofactor-competitive inhibitor SAH. a Structure of SAH. In b, the curves were fitted with equation 2 to generate the values in Table S3A. Each curve used 10,000 data points, but only 50 data points are shown. Each curve is the average of 4 to 6 replicates. c, d represent the relationships of a·k₁ and b·k₂ with inhibitor concentrations (values listed in Table S3B). In D, the IC₅₀ was calculated using equation 1. The reaction conditions used for all the experiments were [PRMT1] = 0.2 µM, [SAM] = 3.5 µM, and [H4FL] = 0.4 µM, with varying concentrations of SAH