Fig. 6
From: Detection of PRMT1 inhibitors with stopped flow fluorescence
Stopped flow fluorescence assay of the substrate-competitive inhibitor H4R3Me2a. a Illustration of H4R3Me2a. In b, the curves were fitted with equation 2 to generate the values in Table S5A. Each curve used 10,000 data points, but only 50 data points are shown. Each curve is the average of 4 to 6 replicates. c and d represent the relationships of a·k1 and b·k2 with inhibitor concentrations (values listed in Table S5B). In c and d, the IC50 was calculated using equation 1. The reaction conditions used for all experiments were [PRMT1] = 0.2 µM, [SAM] = 3.5 µM, and [H4FL] = 0.4 µM, with varying concentrations of H4R3Me2a
