Fig. 7
From: Detection of PRMT1 inhibitors with stopped flow fluorescence
Stopped flow fluorescence assay of DB75. a Structure of DB75. In b, the curves were fitted with equation 2 to generate the values in Table S6A. Each curve used 10,000 data points, but only 50 data points are shown. Each curve is the average of 4 to 6 replicates. In c and d, the IC50 was calculated using equation 1. The reaction conditions used for all experiments were [PRMT1] = 0.2 µM, [SAM] = 3.5 µM, and [H4FL] = 0.4 µM, with varying concentrations of DB75
