Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Increased mtDNA copy number promotes cancer progression by enhancing mitochondrial oxidative phosphorylation in microsatellite-stable colorectal cancer

Fig. 3

Increased mtDNA copy number inhibited apoptosis of MSS CRC cells. a, b Apoptosis analysis by flow cytometry in both SW480 and Caco-2 cells with treatment as indicated. Cells stably transfected with EV or TFAM vector were also treated with CCCP (150 μM) for 4 h before apoptosis analysis. c Western blot analyses for protein levels of caspase 3/9 and cytochrome C (Cyt C) in CRC cells with the indicated treatments. Cells stably transfected with EV or TFAM vector were also treated with CCCP (150 μM) for 4 h before analysis. β-Actin and Cox4 were used as loading controls for cytoplasm and mitochondria, respectively. Cyto cytoplasm, Mito mitochondria. d TUNEL staining in tumor tissues of nude mouse xenograft models developed from different CRC cells stably transfected with different expression vector. Blue: Hoechst 33342; Green: TUNEL-positive nucleus. The data shown are the mean ± SEM from three separate experiments. **P < 0.01

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