Fig. 4

LIN28B binds to mRNAs associated with the DNA damage pathway. a RNA-IP microarray technology was used to identify genome-wide LIN28B-binding RNAs in ovarian cancer cells. b Dot plot (left) showed the enrichment fold of candidate RNA-binding partners of LIN28B in LIN28B RNA-IP compared to control IgG-IP samples. A total of 1555 mRNA transcripts enriched in the LIN28B RNA-IP sample were common in A2780 (total 2549) and TOV-112D (total 3845) cells (right). c GO analysis was performed on the transcripts bound by LIN28B. The major pathways identified by GO were ribosomal biogenesis, protein metabolism, DNA damage, and cell cycle pathways. d mRNA candidates from the DNA damage pathway enriched in LIN28B RNA-IP microarray were validated by independent RNA-IP experiments followed by qRT-PCR detection. The average data of two independent experiments with similar results are shown