Fig. 6

USP7 inhibition induces apoptosis in T-ALL cells. a T-ALL cell lines (JURKAT, MOLT-4, CUTLL1, and CCRF-CEM) were treated with increasing concentrations of P22077 for 24 h, followed by assessment of cell viability using a CCK-8 assay. The data are presented as the mean ± S.D. (Student’s t-test) of three independent experiments; ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001. b Primary T-ALL cells were isolated and treated with different concentrations of P22077 for 24 h, and the cell viability was monitored via trypan blue staining. The data are presented as the mean ± S.D. (Student’s t-test); ^**p < 0.01, and ^***p < 0.001. c CCRF-CEM and CUTLL1 cells were treated with various concentrations of P22077 for 48 h. Cell apoptosis was determined via flow cytometry using an Annexin-V/PI dual staining assay. Columns (right panel) represent the average percentage of Annexin V-positive cells from three independent experiments, shown as the mean ± S.D. (Student’s t-test); ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001