Fig. 2

MiR-23a directly targets the HOXD10-3’UTR in GBM cells. a U373 and LN443 cells were infected with miR-23a-producing lentivirus or control lentivirus, and the expression levels of miR-23a and HOXD10 mRNA were examined by semi quantitative RT-PCR. GAPDH was utilized as an internal control. b The expression levels of HOXD10 protein were examined by immunoblotting in U373 and LN443 cells with or without forced expression of miR-23a. α-Tubulin was used as a loading control. c Diagram of the luciferase reporter vector fused to the 3’UTR of HOXD10 utilized in the luciferase assay. The sequences of miR-23a and the targeted HOXD10-3’UTR are shown. d Dual luciferase assay. HOXD10-3′UTR luciferase activity were measured in miR-23a-overexpressing U373 and LN443 cells. *P < 0.01 and **P < 0.001 vs. without miR-23a. e The expression levels of sprouty2 mRNA in miR-23a-overexpressing U373 and LN443 cells were examined by semi quantitative RT-PCR. f The phosphorylation levels of ERK were investigated by immunoblotting in the indicated cells. g The cell proliferation of U373 and LN443 cells with or without forced miR-23a was investigated and graphed as the means ± SD. * P < 0.05 vs. control cells