Fig. 3
From: miR-23a promotes invasion of glioblastoma via HOXD10-regulated glial-mesenchymal transition
MiR-23a regulates expression of invasion- and glial-mesenchymal transition (GMT)-related genes via HOXD10. a The expression levels of uPAR, RhoA, and RhoC mRNAs were examined in control and miR-23a-overexpressing U373 and LN443 cells by semi quantitative RT-PCR. GAPDH was utilized as an internal control. b, c In U373 and LN443 cells with or without miR-23a overexpression, the mRNA expression levels of the indicated GMT-related genes were investigated by semi quantitative RT-PCR (b) and real-time RT-PCR (c). *P < 0.05 and **P < 0.005 vs. without miR-23a. d, e The expression levels of miR-23a and the indicated molecules were examined by semi quantitative RT-PCR (d) and immunoblotting (e) in LN443 cells with or without miR-23a and HOXD10 overexpression
