Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Epigenetic drug library screening identified an LSD1 inhibitor to target UTX-deficient cells for differentiation therapy

Fig. 1

Epigenetic drug library screening for compounds specifically promoting the differentiation of Utx-null HSPCs. a Schematic showing the experimental design of epigenetic drug screening: HSPCs were isolated from young adult Utxf/f (WT) or Utxf/f; Mx-1-cre (KO) C57BL/6 mice and then treated with compounds from an epigenetic drug library (Selleckchem, 276 compounds in total) individually. After 72-h treatment, cells were analyzed by flow cytometry with cKit and Mac-1 antibodies. b Summary of drug classification in the epigenetic drug library. The library consists of compounds directly targeting epigenetic enzymes and other related factors. c, d Relative mean fluorescence intensities (MFIs) of Mac-1 (c) and of cKit staining (d), measured by flow cytometry, in Utx WT and KO HSPCs treated with individual compounds from the epigenetic drug library or vehicle. e Relative proportions of cKit+ populations in Utx WT and KO HSPCs treated as in c and d

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