Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Epigenetic drug library screening identified an LSD1 inhibitor to target UTX-deficient cells for differentiation therapy

Fig. 4

Utx deficiency repressed the expression of differentiation-related genes and tumor suppressors, while SP2509 treatment reversed this inhibition. a–f Gene set enrichment analysis (GSEA) of genes in the RPS14_DN. V1_UP gene set (a), the Hallmark_IL2_STAT5_signaling gene set (b), the GO_Inflammatory_Response and the BMI1_DN. V1_UP gene set (c), the p53_DN. V1_DN, and the Hallmark_p53_pathway gene set (d), the Hallmark_Myc_Targets_V1 gene set (e), the GO_Ribosomal_Subunit and the GO_Ribosome_Biogenesis gene set (f) in Utx KO HSPCs compared to that in Utx WT HSPCs, and Utx KO HSPCs treated with SP2509 compared to that in cells treated with vehicle (Veh). g Relative mRNA expression level of Car1, Car2, Gata1, Gata2, Fabp4, and Arg1 in Utx WT or KO HSPCs, measured by qPCR. n = 3. h Relative mRNA expression of Fabp4, Igfbp7, Jag1, Arg1, Epha2, and Bax in Utx KO HSPCs treated with vehicle (Veh) or SP2509. n = 3. Error bars represent s.d. *p < 0.05; **p < 0.01; ***p < 0.001

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