Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Epigenetic drug library screening identified an LSD1 inhibitor to target UTX-deficient cells for differentiation therapy

Fig. 5

SP2509 treatment reversed Utx deficiency inducing the histone H3K4 methylation change on globe and specific differentiation-related or tumor suppressor genes in HSPCs. a Western blotting of H3K4m1, H3K4m2, and H3K4m3 in cell lysates from Utx WT or KO HSPCs treated with vehicle (V) or SP2509 (SP) for 12 h. b Quantification of H3K4m1, H3K4m2, and H3K4m3 levels in a. c–f H3K4m1 peaks on Car1(c), Car2 (d), Gata1(e), and Bax (f) in Utx WT or KO HSPCs measured by ChIP-seq. Data analyzed from Gozdecka et al.44 g H3K4m3 peaks on Bax in Utx WT and KO ES cells measured by ChIP-seq. Data analyzed from Dhar et al.49 h MLL4 peaks on BAX in vehicle or OG86-treated leukemic cells measured by ChIP-seq. Data analyzed from Maiques-Diaz et al.50

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