Fig. 1

PcVps75 cannot form a complex with PcRtt109 in vitro or in vivo. a GST-PcVps75F, GST, and beads were incubated with excess amounts of PcRtt109. All proteins were detected by Coomassie staining. b GFP-Rtt109 and protein A-PcVps75F were expressed either together or separately in the double knockout yeast strain (rtt109Δvps75Δ) and detected with anti-GFP antibody. β-actin served as the loading control