Fig. 7 | Signal Transduction and Targeted Therapy

Fig. 7

From: Structural basis for the acetylation of histone H3K9 and H3K27 mediated by the histone chaperone Vps75 in Pneumocystis carinii

Fig. 7

PcVps75 mutants affect the HAT activity of Rtt109 in vivo. a Tenfold serial dilutions of the indicated strains transformed with PcRtt109 or PcRtt109 with PcVps75-truncated plasmids were grown on SCM-Leu/Ura medium at 30 °C with 1.5 μm CPT. b Whole-cell lysates from rtt109Δ vps75Δ gcn5Δ transformed with different PcVps75 plasmids and PcRtt109 as indicated were analyzed via western blotting using antibodies against H3K9ac, H3K27ac, H3K56ac, and H3 (as loading control)

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