Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Chemical compound cinobufotalin potently induces FOXO1-stimulated cisplatin sensitivity by antagonizing its binding partner MYH9

Fig. 2

Interaction between FOXO1 and MYH9 in NPC cells. a Coomassie brilliant blue staining showed the proteins that interacted with FOXO1 in 5–8F cells and the molecular weights of FOXO1, KRT9 and MYH9. b Co-IP experiments detected the interaction of exogenous FOXO1 and MYH9 in 5–8F cells. c Co-IP experiments detected the interaction of endogenous FOXO1 and MYH9 in 5–8F cells. d Cytosolic colocalization of FOXO1 protein and MYH9 protein in NPC cells is visualized by immunofluorescence staining. Scale bar, 25 μm. e MYH9 and FOXO1 expression after FOXO1 was overexpressed or knocked down is shown by western blot analysis. f FOXO1 and MYH9 expression after MYH9 was knocked down is shown by western blot analysis. g MYH9 mRNA expression is shown after FOXO1 overexpression and knockdown; FOXO1 expression is shown after MYH9 knockdown, and data are normalized to ARF5. One-way ANOVA and Dunnett multiple comparison tests were used for analysis

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