Fig. 3

In vitro cytotoxicity and inhibitory effects of C-DVM on cell migration and invasion abilities in 4T1 cells. a The effect of different concentration of C-DVM and other treatments on cell viability of 4T1 cell line at 48 h measured by CCK-8. Error bars represent the mean ± SD (n = 5) of three independent experiments. ^*P < 0.05, ^**P < 0.01,^***P < 0.005 vs D + V group, ^#P < 0.05, ^##P < 0.01,^###P < 0.005 vs DVM group. b Representative images of migration and invasion assessments of DOX (free doxorubicin), D + V (the same dose of free doxorubicin and vinorelbine), DVM, and C-DVM (2.5 μM) in 4T1 cells. Scale bar represents 100 μm. c Quantification of the absorbance of migration and invasion activity for 4T1 cell line. Data were presented as mean ± SD (n = 5), ^*P < 0.05 vs D + V group