Fig. 3

Enhanced fructose utilization mediated by GLUT3 promotes CRC growth in vitro and in vivo. a, b RT-PCR (a) and western blot analysis (b) showing SLC2A3 expression in RKO and DLD1 cells transfected with the control PCDH retrovirus or PCDH-GLUT3 retrovirus. c Glucose uptake by RKO and DLD1 cells with or without ectopic GLUT3 expression. d Glucose-induced proliferation of RKO and DLD1 cells with or without ectopic GLUT3 expression. e Analysis of the colony formation abilities of RKO and DLD1 cells with or without ectopic GLUT3 expression. f 13C-glucose uptake by RKO xenografts with or without ectopic GLUT3 expression. g, h Subcutaneous tumour growth, xenograft tumour images and tumour weights of xenografts from RKO cells with or without ectopic GLUT3 expression in nude mice