Fig. 1
From: Auranofin mitigates systemic iron overload and induces ferroptosis via distinct mechanisms
Auranofin potently upregulates hepcidin expression in Huh7 cells. aHAMP1 mRNA was measured in Huh7 cells treated for 12 h with 5 μM of the indicated drugs (see Table S1 for details); all 106 drugs caused a significant change in HAMP1 mRNA compared to control-treated cells. bHAMP1 mRNA levels were measured in Huh7 cells treated with dihydro-ergotamine Mesylate (1 μM) or AUR (1 μM) for 12 h. cHAMP1 mRNA was measured in Huh7 cells treated for 12 h with the indicated concentration of AUR or BMP6(50 ng/ml). dHAMP1 mRNA was measured in Huh7 cells treated with 0.5 μM AUR for the indicated time. e Cell viability assay was performed in Huh7 cells treated with the indicated concentration of AUR for either 12 or 18 h. f Huh7 cells were co-transfected with pGL3-HAMP1 and the Renilla reporter construct; 36 h after transfection, the cells were treated with AUR (0.5 μM) or 50 ng/ml BMP6 for 18 h, after which luciferase activity was measured. The mRNA levels in panels a, b and d were normalized to β-ACTIN and are expressed relative to the mean control value. The cell line based in vitro experiments were repeated at least three independent times. Error bars indicate the SEM. The data in a and e were analyzed using the Student’s t-test (*p < 0.05). The data in b–d were analyzed using a one-way ANOVA with Tukey’s post hoc test; groups labeled without a common letter were significantly different (p < 0.05)
