Fig. 4
From: Auranofin mitigates systemic iron overload and induces ferroptosis via distinct mechanisms
AUR activates the IL-6/hepcidin axis via the NF-кB pathway. a, bIL-6 and HAMP1 mRNA were measured in Huh7 cells pretreated with 5 μM BAY11–7082 for 1 h, followed by 0.5 μM AUR for an additional 18 h. The mRNA levels were normalized to β-ACTIN and are expressed relative to the mean control value. c Hepatic P-p65, p65, P-IκB-α, IκB-α, and β-actin proteins were measured in male C57BL/6J mice at the indicated times after receiving an intraperitoneal injection of AUR (5 mg/kg body weight). In the summary graphs, P-p65 and P-IκB-α were normalized to p65 and IκB-α, respectively, and are expressed relative to the 0-h value. The experiments were repeated three independent times. Error bars indicate the SEM. All data were analyzed using a one-way ANOVA with Tukey’s post hoc test, and groups labeled without a common letter were significantly different (p < 0.05)
