Fig. 5
From: Auranofin mitigates systemic iron overload and induces ferroptosis via distinct mechanisms
Long-term treatment with AUR reduces iron burden in male Hfe−/− mice. Male and female 8-week-old Hfe−/− mice (n = 10 mice per group) were given daily intraperitoneal injections of AUR (5 mg/kg) or vehicle (control) for 6 weeks, after which hepatic Hamp1 and Id1 mRNA (a), serum iron and transferrin saturation (b), hepatic iron content (c), Perls’ Prussian blue‒stained liver sections (c), hepatic IL-6, Il-1β, and Tnf-α mRNA (d), renal Epo mRNA (d), P-Stat3, Stat3, P-Smad1/5/8, Smad1, P-Erk1/2, Erk1/2, and β-actin protein (e), and duodenal ferroportin (Fpn) immunohistochemistry (f) were analyzed. The mRNA levels were normalized to β-actin and are expressed relative to the respective control group. In the summary plot in panel e, P-Stat3 protein was normalized to Stat3. Error bars indicate the SEM. *p < 0.05 and N.S., not significant (Student’s t-test)
