Fig. 4

Detection of immune parameters before treatment and 90 days after treatment. a–c Flow cytometry was performed with the 6-color TBNK reagent to detect lymphocytes in the blood. d–f Flow cytometry was performed with the Cytometric Bead Array Human Th1/Th2 Cytokine Kit II to detect cytokines in the blood. Data are shown as individual data points with Box and Whiskers graph (bottom: 25%; top: 75%; line: median; whiskers: min to max). Comparison within groups: *P < 0.05; **P < 0.01; ***P < 0.001; comparison between groups: #P < 0.05; ##P < 0.01; ###P < 0.001. Two-tailed Student’s t test (n = 62)