Fig. 5: FOXC1 depletion induces differentiation of LSCs into skin-like keratinized epithelium.
From: Loss of FOXC1 contributes to the corneal epithelial fate switch and pathogenesis
a PCA of the air-lifting-induced dCESs treated with scrambled shRNA and shFOXC1. b Venn diagram showing the overlap between genes preferentially expressed in the dCESs and those that were downregulated after FOXC1 knockdown in the dCESs. c Heatmap showing normalized TPM values of the selected differentially expressed genes in scrambled shRNA-treated and shFOXC1-treated dCESs. d Immunofluorescence staining of the indicated genes in scrambled shRNA- and shFOXC1-treated dCESs. Scale bar, 100 ÎĽm. e Pie chart showing the percentages of differentially expressed genes, with or without FOXC1 binding, that were induced by FOXC1 knockdown in the dCESs. f Genome browser tracks for FOXC1, H3K4me2, and ATAC enrichment signals across the PAX6 and CLU loci in LSCs. g GO BP analysis of genes upregulated in the FOXC1-depleted dCESs. h GSEA of keratinization pathway in the gene expression matrix of scrambled shRNA- versus shFOXC1-treated dCESs
