Fig. 6: FOXC1 regulates interferon signaling pathways in the corneal epithelium.
From: Loss of FOXC1 contributes to the corneal epithelial fate switch and pathogenesis
a GO BP analysis of the genes downregulated in FOXC1-depleted dCESs. b Downregulated interferon alpha/beta/gamma (IFN A/B/G) signaling pathway genes in FOXC1-depleted dCESs. Node size represents fold change. c GSEA of IFN A/B signaling pathway in the gene expression matrix of scrambled shRNA-treated versus shFOXC1-treated dCESs. d, e Genome browser tracks for FOXC1, H3K4me2, and ATAC signals in LSCs, and RNA-Seq signals in the dCESs around the IRF1, IFNAR1, IFNGR1, and IRF9 loci. f GSSM of the top 15 (fold change) downregulated TFs in FOXC1-depleted dCESs. The cutoff value (0.75) is indicated by the dashed line. These 15 TFs were also highly expressed in the scramble group. g Immunofluorescence staining of IRF1 in adult limbus-cornea. Scale bar, 100 ÎĽm. h Immunofluorescence staining of IRF1 in cornea ulcer tissues. Scale bar, 100 ÎĽm
