Fig. 2

AHA1 is important for the growth and migration of osteosarcoma cells in vitro. a, d Proliferation was examined by MTT assay. b, e Time course of anchorage-independent colony formation of U2R and U2OS cells, and representative images of colony growth after 2 weeks are shown. c, f Cells were cultured in the Transwell® cell culture insert. The number of cells that traversed the filters was counted, and triplicate samples were analyzed. a–f Data are shown as mean ± SD of three independent cultures