Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: AHA1 upregulates IDH1 and metabolic activity to promote growth and metastasis and predicts prognosis in osteosarcoma

Fig. 5

IDH1 overexpression partially reverses the suppressive effects of AHA1 knockdown on the growth and migration of osteosarcoma cells. a WB analysis of IDH1 protein expression in the indicated cells. β-actin was used as the loading control. b The proliferation of the indicated cells was determined by MTT assay. c Time course of anchorage-independent colony formation of U2R and U2OS cells, and representative images of colony growth after 2 weeks are shown. d Representative images of cell migration obtained with the Transwell assay are shown, and the levels of cell migration were quantified. e Cells were subjected to F-actin (red) and DAPI (blue) staining and analyzed under an immunofluorescence microscope. Data are presented as mean ± SD of three independent experiments. Student’s t-test was used for the statistical analysis

Back to article page