Fig. 1
From: CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells
Identification of the interaction and co-localization between CD147 and spike protein. a–c The interaction of CD147 and spike was detected by SPR assay (a), ELISA (b), and Co-IP assay (c). The mouse IgG (mIgG) and rabbit IgG (rIgG) were served as negative controls. d OpNS-EM images of CD147, spike(RBD) and CD147-spike(RBD) complexes. Left panels showed the survey view of the micrograph. Right panels showed 8 class averages were selected from a total of more than 300 class averages which were respectively calculated from a total of 6,681 CD147 particles; 5,073 particles of spike(RBD); 12,426 particles of CD147-spike(RBD) complexes. Scale bars: 10 nm. e The co-localization of CD147 and spike protein was observed by immuno-electron microscope. Virions (orange arrows) were observed in virus-infected Vero E6 cells and lung and kidney tissues from COVID-19 patient. The co-localization of CD147 (20 nm-gold colloid, red arrows) and spike protein (10 nm-gold colloid, yellow arrows) in SARS-CoV-2 infected Vero E6 cells and lung and kidney tissues from a patient with COVID-19. Scale bars: 200 nm
