Fig. 5
From: CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells
CD147 is an alternative receptor for SARS-CoV-2 infection in ACE2-deficient cell types. a No interaction of CD147 and ACE2 was detected by Co-IP assay. The mIgG and rIgG were served as negative controls. b No co-localization was found between CD147 and ACE2 by FRET. The color bar denotes FRET ratio. Scale bars: 10 μm. c No co-localization of CD147-ACE2 and the co-localizations of spike-ACE2 and spike-CD147 were observed by immuno-electron microscope (scale bars: 200 nm) and multicolor immunofluorescence (magnification: ×200) in lung tissues from COVID-19 patient. Spike protein, 10 nm-gold colloid, purple arrows; CD147, 20 nm-gold colloid, blue arrows; and ACE2, 40 nm-gold colloid, green arrows. d Virions (red arrows) were observed in lymphocytes of lung tissues from COVID-19 patient. Scale bars: 500 nm. The localization of spike protein and CD3 was analyzed by multicolor immunofluorescence staining. Magnification: ×200. e The gene expressions of CD147 and ACE2 in CD4+ and CD8+ T cells were detected by real-time PCR (***p < 0.001, two-tailed t-test, mean ± SEM). f The expressions of CD147 and ACE2 in unactivated or activated T cells were detected by real-time PCR (*p < 0.05, Mann–Whitney test, mean ± SEM). g The infection efficiency of SARS-CoV-2 pseudovirus was detected by luciferase reporter assay in unactivated and activated T cells (*p < 0.05, **p < 0.01, ***p < 0.001, two-tailed t-test, mean ± SEM). h SARS-CoV-2 pseudovirus infection of CD4+ and CD8+ T cells was inhibited by Meplazumab (***p < 0.001, two-tailed t-test, mean ± SEM). i The gene expressions of CD147 and ACE2 in Vero E6 and BEAS-2B cells were detected by real-time PCR (***p < 0.001, two-tailed t-test, mean ± SEM)
