Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane (M) protein inhibits type I and III interferon production by targeting RIG-I/MDA-5 signaling

Fig. 1

The SARS-CoV-2 M protein inhibits the induction of IFN-β, IFN-λ1, ISG56, and CXCL10 by SeV infection and poly (I:C) transfection. HEK293T cells cultured in 24-well plates (0.8–1 × 105 cells per well) were transfected with pcDNA6B empty vector (E.V., 500 ng) or the SCV2-M plasmid (500 ng). Twenty-four hours after transfection, cells were stimulated by SeV infection (a) or poly (I:C) transfection (b) as indicated, and at 9 and 12 h after stimulation, the cells were harvested for RNA extraction and subsequent RT-qPCR analysis. Three independent biological replicates were analyzed, the results of one representative experiment are shown, and the error bars indicate the SD values. Statistical significance is shown as indicated. SARS-CoV-2 M protein SCV2-M; hours h

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