Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane (M) protein inhibits type I and III interferon production by targeting RIG-I/MDA-5 signaling

Fig. 3

Subcellular localization of the SARS-CoV-2 M protein. HeLa cells seeded on coverslips in 12-well plates were transfected with the indicated plasmids. Twenty hours later, the cells were fixed, blocked, and then incubated with a rabbit anti-Flag antibody and a mouse antibody against the corresponding organelle marker or the indicated protein (a). HeLa cells in (b) were infected with SeV as indicated for 8 h before fixation, blocking and antibody incubation. Subsequently, the proteins were stained with a fluorescence-labeled secondary antibody. MDA-5 was visualized with a mCherry tag. (Right) Intensity profiles of SARS-CoV-2 M protein (Flag-M) and indicated proteins along the plotted lines, as analyzed by Image J line scan analysis. Nucleus were visualized with DAPI (blue). Confocal imaging results are representative of two independent experiments. Scale bar, 10 μm. MitoRed, mitochondria marker; Calnexin, ER marker; CM130, Golgi marker

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