Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) membrane (M) protein inhibits type I and III interferon production by targeting RIG-I/MDA-5 signaling

Fig. 6

The SARS-CoV-2 M protein suppresses the phosphorylation and nuclear translocation of IRF3. a The SARS-CoV-2 M protein affects the phosphorylation of IRF3 upon SeV infection. HeLa cells seeded in six-well plates were transfected with the empty vector or Flag-tagged SARS-CoV-2 M protein plasmids for 20 h before infection with SeV (50 HA/mL). At the indicated time points, the cells were harvested and processed for immunoblotting with the indicated antibodies. b The SARS-CoV-2 M protein prevents the nuclear translocation of IRF3. HeLa cells seeded on coverslips in 12-well plates were transfected with the Flag empty vector or Flag-tagged SARS-CoV-2 M protein plasmids for 20 h before infection with SeV. After infection for 8 h, the coverslips were removed and processed for immunofluorescence staining with the mouse anti-Flag antibody and a rabbit anti-IRF3 antibody. Scale bar, 10 μm. c Quantification of the percentage of IRF3 in the nucleus upon SeV infection. IRF3 molecules in the nuclei in 50 cells per group were counted, and percentages were calculated. Experiments were performed in triplicate and calculated as the means ± SD. Immunoblotting (a) and confocal imaging (b) results are representative of two independent experiments. Empty vector EV, SARS-CoV-2 M protein SCV2-M, hours h

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