Fig. 3: MYH9 interacts with GSK3β to suppress its expression. | Signal Transduction and Targeted Therapy

Fig. 3: MYH9 interacts with GSK3β to suppress its expression.

From: Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma

Fig. 3

a Coimmunoprecipitation analyses of the interaction between MYH9 and the β-catenin destruction complex in HCCLM3 cells. b Immunofluorescence costaining of MYH9 and the β-catenin destruction complex to indicate the colocalization in HCCLM3 (left) and Huh7 (right) cells. The colocalization of MYH9 and the β-catenin destruction complex is shown by calculating the fluorescence intensities along the red arrow crossing the cytoplasm. c QPCR analyses of GSK3β mRNA levels in MYH9-depleted Huh7 and HCCLM3 cells and control cells. d Immunofluorescence staining of MYH9 and APC or AXIN1 expression and localization in MYH9-silenced Huh7 and HCCLM3 cells and control cells (scale bar indicates 5 μm). e MYH9 and GSK3β expression and localization were detected by immunofluorescence costaining in MYH9-overexpressing Huh7 and HCCLM3 cells and control cells (scale bar indicates 5 μm).

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