Fig. 4: HBX protein interacts with MYH9 and induces its expression. | Signal Transduction and Targeted Therapy

Fig. 4: HBX protein interacts with MYH9 and induces its expression.

From: Silencing MYH9 blocks HBx-induced GSK3β ubiquitination and degradation to inhibit tumor stemness in hepatocellular carcinoma

Fig. 4

a Coimmunoprecipitation and silver staining analyses of HBX-associated proteins in HCC cells. b Coimmunoprecipitation analyses of the interaction between HBX and MYH9 in HBX-overexpressing Huh7 and HCCLM3 cells. c The colocalization of HBX and MYH9 was evaluated by immunofluorescence costaining. The colocalization of HBX and MYH9 was shown by calculating the fluorescence intensities along the red arrow crossing the cytoplasm. d QPCR and western blotting analyses were utilized to measure MYH9 expression levels in HCC cells. e ChIP-seq binding peaks searched using the Cistrome Data Browser. f Bioinformatics tools were adopted to identify c-Jun-binding sites inside the transcriptional regulatory region of MYH9. Chromatin immunoprecipitation analyses g, electrophoretic mobility shift assays h, and luciferase reporter assays i were conducted to elucidate c-Jun binding to the transcriptional regulatory sequences of MYH9. j Chromatin immunoprecipitation analyses were conducted to assess c-Jun binding to the transcriptional regulatory sequences of MYH9 in HCC cells.

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