Fig. 2
SIRT3 knockout mice display inflammation in the heart. a Relative quantification of Icam1, Il6, Mcp1, Socs3, and Sod2 mRNA expression in SIRT3 knockout (KO SIRT3) and wild-type (WT) mice. b Western blot analysis showing the levels of p-IκBαSer32/IκBα in total protein extracts and p65 in nuclear protein fractions (NE) obtained from the same samples. The data are the mean ± SD. c EMSA data showing NF-κB DNA-binding activity in the heart. Ab antibody; IC immunocomplex; NE nuclear extract. Western blot analysis showing the levels of FOS and JUN in cytosolic d and nuclear e protein fractions obtained from heart samples of KO SIRT3 and WT mice. The graphs represent the quantification of adenine phosphoribosyl transferase (Aprt)–normalized mRNA levels a or the normalized quantification of protein levels b, d, and e expressed as a percentage of the control samples ± SD. f EMSA data showing AP-1 DNA-binding activity in the heart. g Representative images of Mason’s trichrome staining and quantification of fibrosis expressed as a percentage of WT samples ± SD in the heart. h Representative M-mode transthoracic echocardiographic images and graphs representing evaluations of EF, FS, LV EDD, and LV ESD diameters, and IVRT. The Data are presented as the median ± interquartile range and were compared using the Mann–Whitney test. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT
