Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: SIRT3-mediated inhibition of FOS through histone H3 deacetylation prevents cardiac fibrosis and inflammation

Fig. 6

SIRT3 attenuates FOS/AP-1 signaling via H3K27 deacetylation at the chromatin. Western blot analysis showing the protein levels of acetylated histone H3 in nuclear protein extracts obtained from human AC16 cardiac cells transfected with a LacZ-carrying or SIRT3-carrying plasmids or b scrambled siRNA (siRNA control) or SIRT3 siRNA. The graphs represent the quantification of protein levels normalized to lamin B expressed as a percentage of the control samples ± SD. ChIP assay for the determination of H3K27 acetylation levels at the FOS promoter in human AC16 cardiac cells transfected with LacZ or SIRT3-carrying plasmid c, scrambled siRNA (siRNA control) or SIRT3 siRNA d. The data are the mean ± SD, and comparisons were performed by unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. LacZ a and c or siRNA control b and d

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