Fig. 4
D609 inhibits SI-induced oxidative cell damage by upregulating the MT protein family. a GO analysis of the upregulated genes between the SI-cotreated D609 group vs. the SI-treated group from ftRPE RNA-Seq data. b Heatmaps of the MT family genes at the transcriptional level. c Left panel: Western blot analysis of MT in the ftPRE cells treated with D609 and/or SI for 12 h; β-actin was used as the loading control. Right panel: quantification of the western blots. Data are shown as the mean ± SD. d Immunostaining images of MT in the ftRPE cells treated with D609 (10 μM) and/or SI (10 μM) at 18 h. (e) Phase-contrast images of the ftRPE cells after RNA interference of MT and treatment with D609 (10 μM) and/or SI (10 μM) for 18 h; scrambled siRNA was used in the Mock group. f Phase-contrast bright field (BF), crystal violet (CV) staining and GFP images after D609 (10 μM) and/or SI (10 μM) treatment in the MT2A and MT1G-overexpressing ARPE-19 cells. *p < 0.05. n = 3
