Fig. 5

NSCLC exosomes increased the protein levels of integrin α2β1 by reducing miR-34c-3p. a NSCLC exosomes reduced the expression of miR-34c-3p. Cellular miR-34c-3p levels were detected by quantitative RT-PCR after treatment with NSCLC exosomes. b NSCLC exosomes increased the protein levels of integrin α2β1 in NSCLC cells. Western blotting was performed with the indicated antibodies, and GAPDH was used as a control to normalize levels. c NSCLC exosomes did not affect the mRNA levels of integrin α2β1 in NSCLC cells. The mRNA levels of integrin α2β1 in cells were detected by quantitative RT-PCR after treatment with NSCLC exosomes. d Luciferase activity by luciferase reporters carrying the 3′-UTR of ITGA2 or ITGB1 or mutant ITGA2 or ITGB1 in which the binding site for miR-34c-3p was mutated. The vectors were introduced into 293 T cells along with a negative miR-control (NC) or miR-34c-3p. e Alignment of ITGA2 and ITGB1 3′-UTRs with miR-34c-3p. f The results of coimmunoprecipitation reflect the fact that integrin α2 interacts with integrin β1 in NSCLC cells. ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and n ≥ 3. ITG A2 Integrin α2, and ITG B1 Integrin β1