Fig. 1
From: A novel role of LRP5 in tubulointerstitial fibrosis through activating TGF-β/Smad signaling
Levels of LRP5 are upregulated in the renal tubules of CKD. a, c Western blot analyses and (b, d) densitometry quantification of LRP5, collagen III, and collagen I in the kidney homogenates from 3-month-old Akita and 6-month-old db/db mice, as well as their respective age- and genetic background-matched, non-diabetic controls (n = 8–10). e Immunohistochemical staining of LRP5 (brown color) in the kidneys of Akita and db/db mice and their respective non-diabetic controls (n = 5–6; scale bar = 100 μm). The lower panels are enlarged images of the boxed areas in the upper panels. f Western blot analyses and (g) densitometry quantification of LRP5, α-SMA, and CTGF in the kidney homogenates from WT mice with sham surgery or UUO at day 5 and day 10 post-surgery (n = 5–6). h Immunohistochemical staining of LRP5 (brown color) in the kidneys from WT mice with sham surgery or UUO at day 5 and day 10 (n = 5; scale bar = 75 μm). The lower panels are enlarged images of the boxed areas in the upper panels. Each lane represents an individual mouse. All values are expressed as mean ± SEM. *p < 0.05; **p < 0.01, by unpaired Student’s t-test
