Fig. 3

Knockout of Lrp5 attenuates TGF-β/Smad signaling activation in renal tubules. a Western blot analyses, (b) densitometry quantification and (c) ELISA of total TGF-β1 in the kidneys from WT and Lrp5^−/− mice at day 10 post-surgery (n = 6–8). d Western blot analyses and (e) densitometry quantification of p-Smad2/3, total Smad2/3, TβRI and TβRII in the kidneys from WT and Lrp5^−/− mice at day 10 post-surgery (n = 5–7). f Immunostaining of TβRI (red color; scale bar = 100 μm) and α-SMA (green color; scale bar = 100 μm) in the kidney sections from WT and Lrp5^−/− mice at day 5 post-surgery (n = 5–7), and of Smad2/3 (red color; scale bar = 25 μm), and (g) quantification of nuclear Smad2/3 in the kidney sections from WT and Lrp5^−/− mice at day 10 post-surgery (n = 5–10). h Luciferase activity measurement in the kidney homogenates from WT/SBE-Luc and Lrp5^−/−/SBE-Luc mice at day 10 post-surgery (n = 5–6). Each lane represents an individual mouse. All values are expressed as mean ± SEM. **p < 0.01; n.s., not significant, by two-way ANOVA with pair-wise multiple comparisons