Fig. 6
From: A novel role of LRP5 in tubulointerstitial fibrosis through activating TGF-β/Smad signaling
LRP5 promotes the basal membrane presentation and ligand-induced internalization of TβRs. a, c, f, h Western blot analyses and (b, d, g, i) densitometry quantification of TβRI, TβRII, and LRP5 in the cell factions from HKC-8 cells after transfection with (a, b, f, g) a control plasmid (pcDNA3) or a plasmid expressing LRP5, and (c, d, h, i) a control plasmid (sh-scramble) or a plasmid expressing shRNA for LRP5 (shLRP5) for 48 h, followed by the treatment of 2 ng/ml TGF-β1 for the indicated times. Cells were fractionated and immunoblotted for TβRI, TβRII, and LRP5, with pan-cadherin and α-tubulin as loading controls (n = 3). e Immunostaining of internalized TβRI, TβRII, and LRP5 in HKC-8 cells after transfection of pcDNA3 or a plasmid expressing LRP5 together with either a plasmid expressing TβRI or TβRII for 48 hr, followed by the treatment of 2 ng/ml TGF-β1 for 7.5 min (green color; scale bar = 10 μm). Representative images are shown from at least three independent experiments. All values are expressed as mean ± SEM. *p < 0.05; **p < 0.01, by unpaired Student’s t-test
