Fig. 4

Ferroptosis contributes to erianin-induced cell death in lung cancer cells. a The cellular ROS level was analyzed by a flow cytometer, *p < 0.05, **p < 0.01. b Intracellular GSH levels in H460 and H1299 cells treated with erianin, *p < 0.05, **p < 0.01. c Intracellular MDA levels in H460 and H1299 cells treated with erianin, *p < 0.05, **p < 0.01. d H460 and H1299 cells were treated with erianin with or without the ROS scavenger NAC for 24 h, and cell viability was assayed, **p < 0.01. e H460 and H1299 cells were treated with erianin with or without the ROS scavenger GSH for 24 h, and cell viability was assayed, **p < 0.01. f Intracellular chelatable iron in H460 and H1299 cells treated with erianin was determined using the fluorescent indicator Phen Green SK (green). g Transmission electron microscopy (TEM) was used to observe ferroptosis in H460 and H1299 cells (original magnification: ×100). h The expression of several key ferroptosis regulators was examined by western blotting. i H460 and H1299 cells were treated with erianin with or without ferroptosis inhibitors for 24 h, and the inhibition of growth was assayed, *p < 0.05