Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Erianin, a novel dibenzyl compound in Dendrobium extract, inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis

Fig. 5

Calcium/calmodulin signaling contributes to erianin-induced ferroptosis in lung cancer cells. a The most likely predicted targets of erianin. b Heatmap showing the mRNA analysis of Ca2+/CaM signaling. c The intracellular Ca2+ concentration in H460 and H1299 cells treated with erianin was detected according to the fluorescence intensity of the calcium indicator Fluo-3/AM with fluorescence microscopy. d The intracellular Ca2+ concentration in H460 and H1299 cells treated with erianin was detected by measuring the fluorescence intensity of the calcium indicator Fluo-3/AM with a flow cytometer. e The expression of CaM was examined by western blotting after treatment with erianin for 24 h. f The effect of blocking Ca2+/CaM signaling with ruthenium red on erianin-induced ferroptosis in lung cancer cells after treatment for 24 h. g The effect of blocking Ca2+/CaM signaling with ruthenium red on erianin-induced inhibition of growth of lung cancer, *p < 0.05

Back to article page