Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Hypoxia-induced GBE1 expression promotes tumor progression through metabolic reprogramming in lung adenocarcinoma

Fig. 4

GBE1 induces metabolic reprogramming in LUAD cells. a Seahorse metabolic analysis of ECARs and OCRs in the shGBE1 and shNC A549 cells. b Intracellular 2-NBDG accumulation evaluation in the shGBE1 and shNC A549 cells. c, d The effect of GBE1 knockdown in the A549 cells on lactate production (c) and ATP secretion (d). e The correlation between GBE1 expression levels in the LUAD tissues with Glut1 and LDHA expression levels in the samples in the TCGA data set. f ROS expression in the shGBE1 and shNC A549 cells was analyzed by fluorescence imaging and flow cytometry. g The ratio of NADP/NADPH in the shGBE1 and shNC A549 cells. h RNA-seq and i pan-metabolomic analysis of GBE1 knockdown in the A549 cells. j Heat map showing the fold changes of differentially expressed genes based on the glucose metabolism PCR array. k Schematic illustration of changes in metabolic signaling pathways induced by GBE1 knockdown. Data are represented as the means ± SD. **P < 0.01, ***P < 0.001

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