Fig. 2
From: SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites
PIAS2α is the SUMO E3 ligase for hSSB1. a, b Forty-eight hours after HEK293T cells were transfected with the indicated PIAS plasmids with FLAG-hSSB1, the cells were lysed and the proteins analyzed by western blotting or IP using an anti-FLAG or anti-MYC antibody followed by western blotting, as indicated. c HEK293T cells were lysed with RIPA, and the lysates were subjected to IP using IgG or anti-hSSB1 antibody, as indicated, and were analyzed by western blotting. d HEK293T cells were cotransfected with the indicated plasmids and, after 24 h, were treated with 100 μM etoposide for 24 h, and then lysed and analyzed as in b. Schematic description of the hSSB1 domains (e) and the PIAS2α truncation constructs (f). g Cotransfection of 3MYC-PIAS2α with the indicated hSSB1 plasmids into HEK293T cells for 48 h, followed by cell lysis and analysis as in b. h Cotransfection of FLAG-hSSB1 with the indicated 3MYC-PIAS2α truncations into HEK293T cells for 48 h, followed by cell lysis and were analyzed as in a
