Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Identification and characterization of mammaglobin-A epitope in heterogenous breast cancers for enhancing tumor-targeting therapy

Fig. 1

Screening and evaluation of membrane-associated anti-Mam-A mAbs. a ZR75.1 cells and tumor slides expressing green fluorescent protein (GFP) in the cytoplasm were immunostained by representative mAbs, targeting seven epitopes of Mam-A, and the DiI dye was used as a positive control of membrane staining. b Flow cytometry analyzing the fluorescent intensities of ZR75.1 cells immunostained by mAb785, mAb656 and mAb304. c Representative image of tissue chips stained by different mAbs and the total detecting rate. d Heat maps of Mam-A expression on breast cancer tissue chips stained by different mAbs. e Quantitative analysis of immunostained breast tumor samples on chips by different mAbs (*P < 0.05, **P < 0.01, ***P < 0.001, One way ANOVA /Tukey’s multiple comparisons test was used for multiple group comparison). f Evaluation of mAb specificity by tissue chips, including non-breast cancer tissue and normal tissue chips. MOD, mean optical density; IOD, integrated optical density

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