Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Identification and characterization of mammaglobin-A epitope in heterogenous breast cancers for enhancing tumor-targeting therapy

Fig. 5

Location of mAb785 targeted epitope determination and computer-assisted structure reconstruction. a Three enriched phages were used for the pre-blocking test. Only phage 733 (P733) could completely block mAb785 immunostaining in breast cancer sections (Bar = 100 μm). b The three variant amino acids in peptides carried by phage 733 were highly similar to those in the Mam-A protein. c Replacing the three variant amino acids in the peptides from phage733 did not influence their reactivities with mAb785 (Bar = 100 μm). d Removing the last two amino acids in the peptides did not influence their reactivities with mAb785. e Removing the first two amino acids in the peptides significantly influenced their reactivities with mAb785. f The epitope for mAb785 in Mam-A was determined as the N-terminal 42–51 amino acids

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