Fig. 1

Transcriptome analyses of lung metastases from CRC patients and single-cell RNA sequencing (scRNA-seq) of lung metastases from mouse CRC model. a–f Heatmap representing differential expressed genes (a) and KEGG pathway enrichment analyses in primary tumors from CRC patients with or without distant metastasis (Supplementary Table S1) (c), or that in primary tumors and lung metastases from CRC patients (Supplementary Table S1) (b, d–f). g–j Immunohistochemical staining and semi-quantification of the number of Erbin⁺ stromal cells (g, h) and IgA⁺ (i, j) in primary tumors and lung metastases from CRC patients (Supplementary Table S3). The red dotted line indicates the junction area between tumor area and paracancerous area. IgA⁺ and Erbin⁺ cells per field (×200) in CRC patients (g, j). k, l Immunofluorescence of Erbin⁺ CD19⁺ cells in primary tumors and lung metastases from CRC patients (Table S3), scale bars, 20 μm (k). Semi-quantification of the number of Erbin⁺ CD19⁺ cells by multiple immunofluorescent staining (l). mt-SNE display and graph-based clustering of B220⁺ cells. n Counts of the expression of Erbin in each cluster of B220⁺. o t-SNE display and graph-based clustering of CD38⁺ cells. p Counts of the expression of Erbin in each cluster of CD38⁺