Fig. 5

PD1 expression in IgA+ CD138+ cells in lung metastases of CRC in Erbin-deficient mice. a The flow cytometry analysis of PD1⁺ cells gated on IgA⁺ CD138⁺ cells in lung of tumor-bearing WT and Erbin^−/− mice. b The percentage of PD1⁺ cells in leukocytes of lung, spleen, and blood from tumor-bearing WT and Erbin^−/− mice. c The percentage of PD1⁺ cells gated on IgA⁺ CD138⁺ cells in lung, spleen, and blood of tumor-bearing WT and Erbin^−/− mice. d–f Immunohistochemical staining of IgA and PD1 and semi-quantification of the number of IgA⁺ (e) and PD1⁺ cells (f) in lung metastases from tumor-bearing WT and Erbin^−/− mice. Scale bar, 100 μm. g–j The flow cytometry analysis of PD1⁺ IgA⁺ cells gated (g), the percentage of PD1⁺ cells gated on IgA⁺/IgG⁺/IgM⁺ CD138⁺ cells (h), the percentage of PD1⁺ cells gated on IgA⁺ CD138⁻ cells (i), the flow cytometry analysis of CD5⁺/CD11b⁺ PD1⁺ cells gated on IgA⁺ cells (j), in lung leukocytes of tumor-bearing WT and cKO mice. *p < 0.05 (Student t test)