Fig. 6

The numbers and functions of CD4+ and CD8+ T cells in Erbin-deficient mice. a–h The percentages of CD4⁺ cells (a), CD4⁺ IFNγ⁺ cells (c), CD4⁺ Foxp3⁺ cells (d), CD8⁺ cells (e), CD8⁺ perforin⁺ cells (g), CD8⁺ GrzB⁺ cells (h) and i CD8⁺ IFNγ⁺ cells gated on lung leukocytes in tumor-bearing WT, Erbin^−/−, WT, and cKO mice. The percentages of PD1⁺ cells gated on CD4⁺ cells (b) and CD8⁺ cells (f) in tumor-bearing WT, Erbin^−/−, WT, and cKO mice. j The percentages of CD4⁺ Ki67⁺ and CD8⁺ Ki67⁺ cells gated on lung leukocytes in tumor-bearing WT, and cKO mice. k The flow cytometry analysis of CD8⁺ Ki67⁺ cells gated on lung leukocytes in tumor-bearing WT, and cKO mice. l The flow cytometry analysis of PDL1 expression in MC38-FITC cells, which were co-cultured with spleen B cells isolated from WT and cKO mice for 3 days, respectively. m The cytotoxic activity of tumor cells stimulated by CD8⁺ T cells from WT or cKO mice, B cells from WT or cKO mice, B cells from WT mice co-cultured with CD8⁺ T cells from WT mice, B cells from cKO mice co-cultured with CD8⁺ T cells from cKO mice, and B cells from cKO mice co-cultured with CD8⁺ T cells from WT mice, respectively. n The cytotoxic activity of tumor cells stimulated by the supernatant of B cells from WT or cKO mice, the supernatant of B cells from WT mice co-cultured with CD8⁺ T cells from WT mice, and the supernatant of B cells from cKO mice co-cultured with CD8⁺ T cells from WT mice. o, p Immunohistochemical staining (o) and semi-quantification of the number of Erbin⁺ stroma cells in Norm area, IM area, and CT area (p) from CRC patients (cohort1 and cohort2). Erbin⁺ stroma cells per field (×400). q–t Immunohistochemical staining (q) and semi-quantification of the number of IgA⁺, CD38⁺, and CD8⁺ cells in Norm area, IM area, and CT area (r–t) from CRC patients (cohort1 and cohort2). u Pearson correlation between IgA and Erbin, CD38 and Erbin, CD8 and Erbin in expression in CRC patients (cohort1 and cohort2). *p < 0.05 (Student t test)